Always hold the dropper pointing vertically downwards when taking drops from the bottle. The external surface of dropper should be dry. Avoid bubbles. Failure to follow these precautions may lead to deviations in the test results.
INSTRUCTION
Determinationof Mg2+ + Ca2+
1. Rinse the test tube repeatedly with water to be tested.
2. Using the syringe, place exactly 5 ml of the water to be tested into the test tube.
3. Holding the bottle with the reagent strictly vertical, add the reagent one drop at a time, counting drops.
4. Shake gently after each drop until the colour changes from RED to GREEN.
5. Write down the total number of drops (N1).
DeterminationofCa2+
1. Rinse the test tube repeatedly with water to be tested.
2. Using the syringe, place exactly 5 ml of the water to be tested into the test tube.
3. Hold the bottle with the reagent Ca №1 strictly vertical! Add 5 drops of the reagent Ca №1, seal tube with the cap and shake well for 10 seconds. Wait 1 minute. The test result is not affected by any clouding which may occur.
4. Add one spoon of the reagent Ca №2, seal tube with the cap and shake it well until the solution became PINK.
5. Holding the bottle with the reagent Ca №3 strictly vertical, add the reagent Ca №3, one drop at a time, counting drops. Shake gently after each fall until the colour changes from PINK via VIOLET to BLUE.
6. If there are no changes in to the BLUE colour of the solution after 30 seconds, the analysis is considered completed.
7. Write down the total number of drops (N2).
DeterminationofKH
1. Rinse the test tube repeatedly with water to be tested.
2. Using the syringe, place exactly 5 ml of the water to be tested into the test tube.
3. Add the reagent drop by drop counting them, until color change from blue to red.
4. Each drop is equal to 0.3 German degree of carbonate hardness.
Calculation Mg2+content in sea water (mg/l):
[Mg2+] = 120·N1-12·N2
CalculationCa2+content in sea water (mg/l):
[Ca2+] =20·N2
For example, N1=13, N2=21.
[Mg2+] = 120·13-12·21= 1308 (mg/l)
[Ca2+] =20·21 =420 (mg/l)
Thus, the concentration of magnesium in seawater is 1308 mg/l and the calcium is 420 mg/l.
Immediately after the determination of nitrites levels thoroughly wash the test tube.
For accurate result, temperature of the water to be tested must be at 25±3°С while indoor temperature must not be less than 20°С.
Shelf life: 24 months since manufacture data. Once opened use it within 12 months.